|Chromatography Associates, Inc.
2701 Carolean Industrial Drive
State College, PA 16801
We use test mixes to determine how well we’ve done preparing your
column. We need to know if we have treated the fused silica surface to be
inert and to be compatible with the stationary phase we want to coat.
For inertness we typically use an alcohol such as octanol or decanol to
indicate interaction with active sites on the fused silica surface. Successful
deactivation is indicated by symmetrical peaks with minimal tailing.
Dimethylaniline and dimethylphenol are also often used to indicate
acid/base interactions. Although more important during the packed column
era we still include these.
A hydrocarbon or fatty acid methyl ester is used for calculations of
theoretical plates per meter and coating efficiency.
The test is conducted isothermally as we feel temperature programmed
column testing leads to sharpening peaks which can mask peak tailing (an
inertness problem) and artificially increases efficiencies.